The Effect of Myostatin on Glutamate Excitotoxicity Induced in SH-SY5Y Cell Line

Authors

  • Hicran Felek Sivas Cumhuriyet Üniversitesi Tıp Fakültesi
  • Tunahan Çatal Sivas Cumhuriyet Üniversitesi Tıp Fakültesi
  • Fatih Yulak Sivas Cumhuriyet Üniversitesi Tıp Fakültesi Fizyoloji Anabilim Dalı
  • Ahmet Kemal Filiz Sivas Cumhuriyet Üniversitesi Tıp Fakültesi Fizyoloji Anabilim Dalı
  • Sebahattin Karabulut Sivas Cumhuriyet Üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu

Abstract

Aim: Excitotoxicity is a pathological condition caused by excessive concentrations of glutamate. Myostatin is a myokine expressed by skeletal muscles and little is known about its effects in the brain. In this study, we investigated the effect of myostatin treatment on cellular viability, oxidant-antioxidant status and apoptosis against glutamate excitotoxicity in SH-SY5Y cells.

Method: SH-SY5Y cells were cultured and divided into 4 groups as 1. control group, 2. glutamate group, 3. myostatin group, 4. myostatin + glutamate group. After the treatments were applied, cellular survival was determined by XTT method, Total oxidative stress (TOS), Total antioxidative stress (TAS) and caspase-3 levels were determined.

Results: Glutamate decreased cell survival and TAS levels, but increased TOS and caspase-3 levels. Although myostatin treatment alone partially increased antioxidant levels, it had no significant effect when co-treated with glutamate.

Conclusion: These results suggest that myostatin has no ameliorative effect against cellular damage caused by glutamate excitotoxicity.

Published

30.06.2024

How to Cite

Felek, H., Çatal, T., Yulak, F., Filiz, A. K., & Karabulut, S. (2024). The Effect of Myostatin on Glutamate Excitotoxicity Induced in SH-SY5Y Cell Line. Health Sciences Student Journal, 4(1), 1–6. Retrieved from http://ojs.healthssj.com/index.php/panel/article/view/15