The Effect of Myostatin on Glutamate Excitotoxicity Induced in SH-SY5Y Cell Line
Abstract
Aim: Excitotoxicity is a pathological condition caused by excessive concentrations of glutamate. Myostatin is a myokine expressed by skeletal muscles and little is known about its effects in the brain. In this study, we investigated the effect of myostatin treatment on cellular viability, oxidant-antioxidant status and apoptosis against glutamate excitotoxicity in SH-SY5Y cells.
Method: SH-SY5Y cells were cultured and divided into 4 groups as 1. control group, 2. glutamate group, 3. myostatin group, 4. myostatin + glutamate group. After the treatments were applied, cellular survival was determined by XTT method, Total oxidative stress (TOS), Total antioxidative stress (TAS) and caspase-3 levels were determined.
Results: Glutamate decreased cell survival and TAS levels, but increased TOS and caspase-3 levels. Although myostatin treatment alone partially increased antioxidant levels, it had no significant effect when co-treated with glutamate.
Conclusion: These results suggest that myostatin has no ameliorative effect against cellular damage caused by glutamate excitotoxicity.
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