Investigation of the neuroprotective effect of hydralazine on PTZ-induced neurotoxicity in the SH-SY5Y cell line

Abstract

Objective: Acute seizure is an important neurological disorder that occurs due to
excessive and synchronized discharges of a group of cortical neurons and causes
neuronal loss in the hippocampus, although it is clinically temporary. Oxidative stress
has an effective role in hippocampal damage that occurs after seizures. The aim of this
study is to investigate the neuroprotective effects of hydralazine in an acute seizure
model created with pentylenetetrazole (PTZ) in the SH-SY5Y cell line.

Method: In the SH-SY5Y cell culture, 4 groups were planned as control group, PTZ
group, hydralazine group and hydralazine + PTZ group. While no application was
made to the control group, hydralazine was applied to the hydralazine + PTZ group at
various concentrations (6.25; 12.5; 25; 50 and 100 µg / ml) and PTZ was applied 24
hours later. The effect of our drug on toxicity was determined by XTT cell viability
test. Tissue TOS concentrations in cell supernatants were measured by the automated
test method. Hydrogen peroxide was used for the efficiency of the analysis.

Results: When evaluating XTT cell viability results, hydralazine did not affect
neuronal viability in toxicity induced SH-SY5Y cells. In addition, hydralazine did not
have a significant effect on TOS levels (P>0.05).

Conclusions: The data obtained showed that hydralazine at the doses we studied did
not have a neuroprotective effect on the SH-SY5Y cell line neurotoxicity induced by
PTZ.