Investigation of the Effect of Galium Aparine Extract on Glutamate Excitotoxicity Induced in C6-Glioma Cell Line

Abstract

Aim: Supraphysiological concentrations of glutamate, the brain's main excitatory neurotransmitter, leads to glutamate excitotoxicity. Galium aparine (GA) is a plant known as a "yogurt herb" in Anatolia and is used in the treatment of diseases such as jaundice, cancer, and epilepsy. This study investigated the effects of GA treatment on cellular viability and oxidant-antioxidant capacity against glutamate neurotoxicity in C6 cells. Method: C6-glioma cells were cultured and 4 groups were divided into 1. control group, 2. glutamate group, 3. Galium aparine group, 4. Galium aparine + glutamate group. The effects of the treatments administered in all control and experimental groups on neuronal survival, Total oxidative stress (TOS), and Total antioxidative stress (TAS) levels in the C6 cell line were investigated. Results: The reducing effect of glutamate cytotoxicity on cell survival was ameliorated by GA. In addition, the increase in TOS induced by excitotoxicity was decreased by GA treatment. In addition, GA increased cellular antioxidant levels. Conclusion: These results indicate that GA has a neuroprotective effect against cellular damage caused by excess glutamate and that the improvement in oxidant-antioxidant balance is involved in this effect.