Investıgatıon of Neuroprotectıve Effect of Ethosuximide Agaınst Hydrogen Peroxıde-Induced Oxıdatıve Damage in The C6 Glıal Cell Line

Abstract

Current studies have revealed that Etosuksimide has positive effects on the nervous system. However, its effect on hydrogen peroxide-induced oxidative stress in glial cells is still unclear. In this study, we aimed to examine the effect of ethosuximide on glial damage after hydrogen peroxide-induced oxidative damage in C6 glial cells. C6 glioma cells were employed in the study. Cells in the H₂O₂-treated group were exposed to H₂O₂ for a 24-hour period. The ethosuximide group was incubated with different concentrations of ethosuximide for 24 hours. Cells in the combined ethosuximide and H₂O₂ group underwent a one-hour incubation with ethosuximide, followed by a 24-hour exposure to H₂O₂. Cell viability was evaluated using the XTT assay. Flow cytometry was utilized to assess the impact on apoptosis. When comparing the ethosuximide and H₂O₂ group to the H₂O₂-only group, it was found that ethosuximide did not alter cell viability or apoptosis patterns in C6 cells. In summary, ethosuximide did not demonstrate a significant protective effect against hydrogen peroxide-induced oxidative stress and apoptosis in the C6 cell line. This result may be attributed to insufficient dosage or incubation time, or a combination of both.