The Protective Role of Sodium Hydrogen Sulfide (NaHS) Against Glutamate Toxicity in the C6 Glioma Cell Line

The Neuroprotective Effects of NaHS

Abstract

Objective: Glutamate, the primary excitatory neurotransmitter in the central nervous system, plays a vital role in normal neural processes. However, excessive accumulation of glutamate can lead to neuronal damage through excitotoxicity. Although hydrogen sulfide (H₂S) has been reported to have various neuroprotective effects, its role in glutamate-induced toxicity remains unclear. This study aimed to investigate the potential protective effects of sodium hydrogen sulfide (NaHS) against glutamate excitotoxicity in a C6 glioma cell line.

Methods: C6 glioma cells were cultured and divided into four groups: (1) Control, (2) Glutamate, (3) Sodium Hydrogen Sulfide, and (4) Sodium Hydrogen Sulfide + Glutamate. The effects of treatments on cell viability were assessed using the XTT assay.

Results: NaHS did not prevent the glutamate-induced reduction in cell viability (p>0.05).

Conclusion: These findings suggest that NaHS does not exert neuroprotective effects against glutamate-induced cellular damage.