Investigation of the Effect of Passiflora Incarnata on the Glutamate Excitotoxicity Model Induced in C6 Glioma Cells

Abstract

Aim: Glutamate is the primary neurotransmitter with excitatory properties in the central nervous system (CNS), and its excessive increase leads to the pathological condition known as glutamate excitotoxicity. Passiflora incarnata (PI), also known as passion flower, is a therapeutically promising plant that stands out with its sedative, anti-anxiety and anti-epileptic effects. In this study, the effect of PI application on cell viability and total oxidant-antioxidant system against glutamate excitotoxicity in C6 cells was investigated.

Method: After C6-glioma cells are cultured; Four separate groups were created: 1.Control group, 2.Glutamate group, 3. Passiflora incarnata group, 4. Passiflora incarnata + Glutamate group. The groups were evaluated in terms of neuronal survival, Total oxidative stress (TOS) and Total antioxidative stress (TAS) levels by XTT cell viability measurement. Data were analyzed by One-way ANOVA method using SPSS software. Statistical significance was accepted as p<0.05.

Results: No significant effect of Passiflora incarnata was observed on the effect of glutamate on reducing cell survival. No antioxidative effect of PI was observed on TOS levels, which increase with glutamate excitotoxicity. Additionally, PI did not change cellular antioxidant levels at a statistically significant level.

Conclusion: These results show that PI does not have a neuroprotective effect against the cellular damage caused by glutamate excitotoxicity, and does not cause any change in the total oxidant-antioxidant system.